레몬밤 추출물의 항산화 효과 및 3T3-L1세포의 지방 축적 억제 효과

Abstract

Obesity is increasing due to various factors such as westernized eating habits and stress. Obesity refers to the accumulation of excessive amounts of fat in the subcutaneous layer and body tissues due to an increase in the number or size of fat cells. Therefore, In this study, the anti-obesity activity and antioxidant activity were confirmed through a study on the differentiation of 3T3-L1 preadipocytes into adipocytes using lemon balm extract, which has an effect on the reduction of adipocytes. Lemon balm powder was shown to have antioxidant activity by scavenging DPPH radicals. In order to check the effect of lemon balm extract on adipocyte differentiation inhibitory activity and adipogenesis, 3T3-L1 preadipocytes were treated by concentration while inducing differentiation. In this study, we found that lemon balm extract reduced the concentration-dependently differentiating 3T3-L1 preadipocytes into adipocytes. To investigate the mechanism of action for this activity, peroxisome proliferator-activated receptor γ (PPARγ), CCAAT-enhancer-binding protein α (C/EBPa), and Sterol regulatory element-binding proteins that play an important role in adipocyte differentiation (SREBP-1) The activity of genes involved in adipocyte differentiation was confirmed. As a result of the experiment, lemon balm extract decreased the expression of genes and proteins of PPARγ, C/EBPa, and SREBP-1, and suppressed the expression of adipogenesis marker genes involved in adipogenesis. In addition, the activities of Catalase and SOD, which are genes related to antioxidants, were checked. According to their results, it was found that lemon balm ethanol extract inhibited adipocyte differentiation or had anti-obesity activity linked to antioxidant activity.