규조류 Chaetoceros curvisetus를 감염하는 살조류 바이러스(CcurV)의 분리 및 특성

Abstract

Approximately 20 years ago, viruses or virus like particles (VLPs) in the ocean were reported. Since then, about 50 species of algae viruses or VLPs have been reported up to now and have turned out to exist in various hosts such as Chlorophyceae, Prasinophyceae, Raphidophyceae, Bacillariophyceae, Dinophyceae, Pelagophyceae, Phaeophyceae, Haptophyceae etc. This study conducted a research on morphological, physiological features of a virus infecting a diatom (Bacillariophyceae) Chaetoceros curvisetus, which was separated in Korea. The virus was isolated from a sediment sample collected from a sampling station in March 2011 in Jaran Bay, Gyeongsangnam-do. The new algicidal virus infecting C. curvisetus was identified from Korean coastal waters and was named CcurV. C. curvisetus infecting virus (CcurV), which lysed the diatom C. curvisetus, was partially characterized. CcurV is a ca. 30 ± 5 ㎚ in diameter, of an icosahedral virus, and replicates within the host's cytoplasm. The latent period was estimated to be between 36 to 72 hours. When intra·interspecific infection characteristics of CcurV were checked with 20 different algae strains, it turned out to infect only C. curvisetus. The thermal stability test showed that the best stability was maintained at 4℃. The cryopreservation test showed that the best concentration of cryoprotectant was 10% DMSO. These are primary results of researches on the characteristics of CcurV, e.g. viral sizes, shapes, and other physiological characterizations. Therefore, further studies are expected to identify complete viral sequences to gain insight into the gene functions of CcurV.