LPS로 유도된 RAW 264.7 대식세포 염증반응에서 미역 에틸아세테이트 분획물의 항염증 효과

Abstract

Undaria pinnatifida ethanolic extract was fractionated with several solvents. Among the fractions, the ethyl acetate fraction showed the highest inhibitory activity on LPS-induced nitric oxide (NO) production in RAW 264.7 macrophage cells. To further investigate the mechanism underlying its anti-inflammatory effect, Undaria pinnatifida ethyl acetate extract (UPE) was used in this study. UPE was investigated via in vitro inhibitory activities against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 murine macrophage cells. The results indicate that UPE significantly inhibited LPS-stimulated NO production in a dose-dependent manner and suppressed the expression of inducible nitric oxide synthase (iNOS) with no cytotoxicity. Pro-inflammatory cytokines were also reduced by the UPE pretreatment in LPS-stimulated RAW 264.7 cells. Treatment of UPE strongly suppressed nuclear translocation of NF-κB by preventing proteolytic degradation of inhibitor of κB (IκB)-α in LPS-stimulated RAW 264.7 cells. Moreover UPE inhibited the phosphorylation of Akt and mitogen-activated protein kinase (MAPK) in LPS-stimulated RAW 264.7 cells. These results indicate that the UPE may contain anti-inflammatory compounds and it can be used as a source of functional food to prevent inflammatory deseases.