자가영양과 혼합영양 배양에 따른 진안점조류 Monodus subterraneus와 Nannochloropsis oceanica의 성장 및 영양조성 비교

Abstract

Eicosapentaenoic acid (EPA) is one of the most important n-3 highly unsaturated fatty acids (HUFA). EPA produced from marine organisms is widely used in nutraceuticals. Freshwater Eustigmatophyceae Monodus subterraneus is known to have high amount of EPA compared to marine Eustigmatophyceae Nannochloropsis oceanica. In this study, the optimum culture conditions such as media, temperature, light in autotrophic culture condition for M. subterraneus are investigated. The growth and composition of nutrient as amino acids and fatty acids of M. subterraneus were also examined and compared to those of N. oceanica. And the dietary value of these microalgal species for rotifer Brachionus plicatilis was tested. The optimum media, temperature, and light intensity for M. subterraneus in autotrophic culture were JM media, 25℃, and 80 µmol photons m-2 s-1, respectively. The growth, cell size and weight, and biomass of M. subterraneus cultured with glucose 2% in mixotrophic condition were higher than those in autotrophic condition. With regard to nutrient composition, M. subterraneus cultured in autotrophic condition showed higher contents of amino acids and fatty acids than N. oceanica in autotrophic condition did. And those of M. subterraneus cultured in mixotrophic condition were the lowest. The dietary value for B. plicatilis fed M. subterraneus cultured in autotrophic condition was better than that fed N. oceanica.