PUKYONG

Dual cross-linked polyvinyl alcohol/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid hydrogel, an antioxidant and antimicrobial wound dressing

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Alternative Title
이중 가교폴리비닐알코올/ 메타크릴레이트화 히알루론산/ 키토올리고당-시나프산 하이드로겔, 항산화 및 항균 상처 드레싱
Abstract
항산화 및 항균 효능과 적절한 기계적, 생물학적, 물리적 특성을 지닌 상처 드레싱용 하이드로겔은 상처 치유 응용 분야에서 큰 관심을 받고 있으나, 이에 대한 개발은 여전히 중요한 과제로 남아 있습니다. 이에 본 연구에서는 항산화 및 항균 효능을 지닌 전층 피부손상용 상처 드레싱으로 키토올리고당(COS)-시나핀산(SA)(COS-SA) 유도체를 함유한 이중 가교 폴리(비닐 알코올)(PVA)/메타크릴레이트 히알루론산(HAMA) 복합 하이드로겔을 제작하였습니다. 이후 제작된 하이드로겔의 화학적, 물리적, 기계적, 항산화제, 항균성, 시험관 내 생체 적합성 및 생체 내 상처 치유 효능에 대해 연구하였습니다. 본 연구를 통해 제작된 복합 하이드로겔은 우수한 유체 흡수성과 적절한 기계적 안정성을 가진 균일한 다공성 구조를 나타냈으며, COS-SA 유도체의 첨가로 인해 하이드로겔의 체외 생체 적합성, 항산화 및 항균 효능이 현저히 증가하였습니다. 이것은 생체 내 상처 치유 과정을 크게 향상시켰으며, 이는 COS-SA유도체를 함유한 PVA/HAMA 하이드로겔 드레싱이 상처 치유 응용 분야에 상당한 잠재력을 가지고 있음을 시사합니다.
Author(s)
SRI RANGA DENIYE DON PATABENDIGE MILAN
Issued Date
2022
Awarded Date
2022. 8
Type
Dissertation
Publisher
부경대학교
URI
https://repository.pknu.ac.kr:8443/handle/2021.oak/32684
http://pknu.dcollection.net/common/orgView/200000642844
Affiliation
Pukyong National University, Graduate school
Department
대학원 4차산업융합바이오닉스공학과
Advisor
Won-Kyo Jung
Table Of Contents
Chapter 1. The overview of skin anatomy, wound healing, and antioxidant and antimicrobial properties of wound dressings for skin tissue regeneration 1
1. Introduction 2
1.1. Anatomy of skin 2
1.2. Wound 4
1.3. The classical wound healing process 7
1.4. Skin wound microbiology 10
1.5. Requirements of ideal wound dressings and substitutes 12
1.6. Hydrogels for wound dressing 19
1.7. Antibacterial properties 20
1.8. Antioxidant properties 26
Chapter 2. Fabrication and characterization of Dual cross-linked polyvinyl alcohol/methacrylate hyaluronic acid/chitooligosaccharide-sinapic acid hydrogel 28
Abstract 29
1. Introduction 31
2. Materials and methods 34
2.1. Materials 34
2.2. Synthesis of COSs-SA 35
2.3. Characterization of COSs-SA 35
2.4. Antioxidant activity of COSs-SA conjugate 36
2.5. Antimicrobial activity of COSs-SA conjugate 36
2.5.1. Microbial culture conditions 36
2.5.2. Screening of antimicrobial properties of COSs-SA conjugates 38
2.6. Cytotoxicity of COSs-SA conjugate 39
2.7. Synthesis of HAMA 39
2.8. Preparation of PVA/HAMA/COS-SA hydrogel 40
2.9. Characterization of the cross-linked hydrogel 41
2.9.1. Microstructural characterization 41
2.9.2. Porosity 41
2.9.3. FTIR analysis 42
2.9.4. X-ray differentiation analysis (XRD) 42
2.9.5. Thermal properties 42
2.9.6. Gel fraction 43
2.9.7. Swelling properties 43
2.10. Mechanical properties 43
2.10.1 Rheological measurements 43
2.10.2. Compressive strength 44
2.10.3. Intracellular ROS scavenging by hydrogel 44
2.11. Antimicrobial activity of hydrogel 44
2.12. In direct and direct cell cytotoxicity 45
2.13. In vivo animal experiment 46
2.14. Hitomopological evaluation 47
2.14.1. H & E staining 47
2.14.2. Masson’s trichrome staining 47
2.15. Statistical analysis 48
3. Results and discussion 48
3.1. Chemical synthesis and characterization of COSs-SA 48
3.2. Antioxidant properties of the COS-SA conjugate 55
3.3. Antimicrobial properties of COS-SA 57
3.4. Cytotoxicity of COSs-SA conjugate 60
3.5. Characterization of HAMA 62
3.6. Characterization of hydrogel 64
3.6.1. Mechanical properties of hydrogel 64
3.6.2. Microstructural characterization and porosity 67
3.6.3. Gel fraction, swelling, and water retention properties 71
3.6.4. FT-IR spectroscopy 74
3.6.5. X-ray diffraction and thermal properties 76
3.7. Intracellular ROS scavenging by hydrogel 81
3.8. Antimicrobial activities of the hydrogel 83
3.9. Indirect and direct cell cytotoxicity 86
3.10. In vivo wound-healing properties 89
3.11. Histomorphological analysis 91
4 . Conclusion 95
5. Reference 96
Acknowledgment 113
Appendix. Curriculum Vitae 114
Degree
Doctor
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