강도다리, Platichthys stellatus의 혈 액학적 성상, 항산화 및 면역반응에 대한 EGCG 급이의 영향

Abstract

In this study, changes in hematological properties, antioxidant and immune response were analyzed in starry flounder by feeding polyphenol, EGCG, which has been reported to have various physiological activities. EGCG is present in a variety of foods consumed by humans and is one of the main catechins in green tea and one of the natural antioxidants. EGCG concentration 0, 50, 100, 150, 200, 250 mg/ using starry flounder (length 16.4cm, weight 81.8±0.9g) to check the changes in hematological properties, antioxidant and immune response according to EGCG feeding After making the feed in kg, it was fed. After 2 weeks of acclimatization, EGCG feed was fed to each section and sampling was performed at 2 and 4 weeks, respectively. Weight and length were measured before the experiment, and then weight and length were measured at 2 weeks and 4 weeks. was measured and growth factors were confirmed by body weight gain (BWG), specific growth rate (SGR), hepatosomatic index (HSI), and feed effecifi ratio (FER). Upon sampling, the RBC count, Hb concentration, and Ht level were checked using whole blood immediately after samling. By separating the serum, the prganic components blood glucose and total protein, inorganic components calcium and magnesium, and the enzyme components GOT and GPT, non-specific the immune response, lysozyme activity and phagoytosis, were measured. In addition, changes in SOD and CAT activity were confirmed using sampled gill, liver, kidney, and intestinal tissues. As a result of the above experiment, growth factors such as BWG, SGR, and FER showed significant increases, and HSI did not show significant changes. There were no significant changes in RBC, Hb, and Ht. In the case of serum analysis, calcium, magnesium, and total protein were not significantly changed, and glucose was decreased. There were no significant changes in the serum enzyme components GOT and GPT. In the case of antioxidant enzymes SOD and CAT, gills, liver, kidney, and intestine all showed significant increases, and it was confirmed that lysozyme activity and phagocytosis, which are immune responses, were significantly increased. In clusion, EGCG had a positive effect on the growth rate, antioxidant and immune response of starry flounder.