Alternative diagnostic approach and laser treatment for urinary tract infection
- Abstract
- Urinary tract infection (UTI) is an infection in any part of the urinary system, such as the urethra, bladder, ureters, and kidneys. UTIs typically occur when bacteria enter the urinary tract through the urethra and begin to spread in the bladder or any other urinary system. This study aims to develop an alternative diagnosis and laser treatment for patients with urological procedures. The approach to diagnose the bacteria-caused UTI is using anthocyanin from eggplant peels which can identify the color changes from bacteria. For the alternative treatment, a 405 nm laser light is used and delivered using basket integrated fiber device. The diagnostic process is successfully created using anthocyanin, which is able to distinguish bacteria based on the color production of each bacterium. The identification ability of anthocyanin is due to urease production, which impacts the different ammonia levels of bacteria. Additionally, the treatment process also obtained an inhibitory effect. The production of reactive oxygen species attempted to remove the biofilm on the urethral stent. The inhibition rate corresponds to a 2.2 log reduction in colony-forming units/mL of bacteria after 10 minutes of 405 nm light exposure (0.3 W/cm2). Thus, the diagnostic approach and laser treatment application can be used for patients with urological procedures with a further examination in clinical translations.
- Issued Date
- 2023
- Awarded Date
- 2023-02
- Type
- Dissertation
- Keyword
- Urinary tract infection
- Publisher
- 부경대학교
- Affiliation
- Pukyong National University, Graduate School
- Department
- 대학원 4차산업융합바이오닉스공학과
- Advisor
- Hyun Wook Kang
- Table Of Contents
- 1. Introduction 1
1.1. Urinary tract infection 1
1.2. The importance of early diagnosis of UTI 4
1.3. Overview of 405 nm laser as an alternative treatment for UTI 7
2. Development of POC-diagnostic of UTI using anthocyanin extracted from eggplant peels 9
2.1. Purpose 9
2.2. Materials and Methods 10
2.2.1. Anthocyanin extraction 10
2.2.2. Synthesis and characterization of AgNPs 10
2.2.3. Bacterial strain and culture 11
2.2.4. Preparation of anthocyanin-based liquid test 11
2.2.5. Image quantitative analysis 12
2.2.6. Development of mobile application based on convolutional neural network 12
2.3. Results 16
2.3.1. Anthocyanin color pH variation 16
2.3.2. Anthocyanin conjugated with AgNPs 18
2.3.3. Determination of bacterial detections and color responses against anthocyanin 20
2.3.4. Mobile application for bacterial classification 23
2.4. Discussion 26
3. Inhibitory effect of 405 nm laser light on bacterial biofilm on urethral stent 28
3.1. Purpose 28
3.2. Materials and methods 29
3.2.1. Urethral stent 29
3.2.2. Bacterial biofilm formation 29
3.2.3. Experimental setup 31
3.2.4. MTT assay 31
3.2.5. Bacterial viability 32
3.2.6. Evaluation of oxidative stress 33
3.2.7. DPPH scavenging activity 34
3.2.8. Protein leakage 34
3.2.9. Microscopic evaluation of biofilm formation 34
3.2.10. Statistical analysis 35
3.3. Results 36
3.3.1. Bacterial culturing in US 36
3.3.2. Evaluation of effect 405 nm laser in human cell lines (CCD-986sk) 36
3.3.3. Evaluation of bacterial inhibition in US 36
3.4. Discussion 46
4. Conclusion and further directions 49
5. References 50
6. Acknowledgments 54
- Degree
- Master
- Appears in Collections:
- 대학원 > 4차산업융합바이오닉스공학과
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