Fish-Gelatin과 Fucoidan을 이용한 Microparticle의 항암제 전달물질로서의 특성 분석

Abstract

The Drug Delivery System aims to efficiently deliver drugs, including anticancer drugs, to the desired target when necessary to reduce side effects caused by the drug and to improve the therapeutic effect of symptoms by maximizing efficacy. When a drug is directly delivered to a target molecule in a drug delivery system, a polymer or nanocapsules are largely used. Recently, more and more studies on biocompatibility of biopolymers have been continuously conducting. Gelatin, an important polymer derived from collagen, has high biocompatibility and biodegradable properties and is used in a wide range of fields such as food, medicine, and cosmetics due to its functional and technical characteristics. In addition to gelatin, fucoidan is a complex sulfate polysaccharide known for its functions, such as anticoagulant, antithrombotic, antitumor activitie… and is used as an edible food in Japan and Korea. Technically, it is known that fucoidan can bind anticancer drugs due to the sulfate ester groups contained in its structure. Therefore, fucoidan is considered as a potential substance for drug delivery systems. In this study, microparticle (GFM) based on fish-gelatin and fucoidan was prepared as a drug delivery system to bind and release Doxorubicin. The results showed that the degree of binding of the Doxorubicin is proportional to the content of fucoidan, which was more pronounced than that of microparticle (GM) composed of gelatin only. In addition, Doxorubicin could quickly bind GFM over time. Importantly, based on the viability of McA RH7777 tumor cell through MTT assay, it was demonstrated that the GFM could be used as a drug delivery system in binding and releasing Doxorubicin.