GSK3β의 발현이 참굴 Pacific oyster, Crassostrea gigas (Magallana gigas)의 에너지원 경로에 미치는 영향

Abstract

Crassostrea gigas is a frequently studied species in understanding physiological processes in bivavle. Similar to other animals, oysters store glucose in the body as glycogen. Glycogen is known to supply energy for germ cell development and maintenance. Therefore, this study aims to examine the effect of GSK3β on the annual cycle of oysters and the glycogen synthesis pathway, and to investigate the energy pathway in comparison with seasonal variation. Oysters were sampled monthly for one year and were subjected to glycogen content, RT-PCR, FISH and western blot analysis. The year-round glycogen content significantly differs only in the mantle edge during spring and summer of both sexes, but not in labial palp, digestive gland, gonad, and adductor muscle. The expression of GSK3β mRNA level was highest in October for female and April for male. Both sexes had the lowest expression in July. In adductor muscle, female and April for male. Both sexes had the lowest expression in July. In adductor muscle, female and male showed the highest expression in April and the lowest in July and October. The pattern of GSK3β expression in gonads and adductor muscle was similarly confirmed while glycogen synthase decreased in May, when the condition index is the highest, and p-GSK3β was decreased in October and July when spawning occurred, while glycogen synthase increased. Overall, it is thought that p-GSK3β expression is high in C. gigas at ripe, which inhibits glycogen synthesis and is used as energy for growth and maturation, and glycogen synthesis occurs for energy storage during degeneration.