다양한 지역에서 유래한 흰다리 새우 (Litopenaus vannamei)에서의 간췌장 바이러스의 검출과 염기서열 비교

Abstract

The Hepatopancreatic parvovirus (HPV) causing Hepatopancreatic parvovirus disease is one of the viruses that has been affecting the shrimp industry for the past 50 years. Hepatopancreatic parvovirus (HPV) diseases in recent years have been found in wild and farmed penaeid shrimps around the different geographical locations of the World. This is an indication that there may be changes that may lead into differences in sequences among shrimps. Currently, there are four complete genome sequences/isolates of HPV that have been reported with a large number of other partial sequences. In this experiment, I collected five different shrimp samples of whiteleg shrimp (Litopenaus vannamei) from five different geographical origins, four locations in South Korea, and one sample from Malaysia. In order to detect the presence of viruses and compare sequences of five groups of whiteleg shrimp with different geographical origins. The collected shrimp samples were subjected to DNA extraction, Polymerase Chain Reaction (PCR), Cloning, Transformation, Quantitative Realtime PCR and Sequencing. The five samples showed to be similar in their sequences to the Korean isolate of HPV strain (FcDNV)