유기 자외선 차단제, Benzophenone-3와 Octinoxate및 수온 조합이 점망둑 (Chaenogobius annularis)의 최종성숙 과정에 미치는 영향

Abstract

2-hydroxy-4-methoxybenzophenone (benzophenone-3, BP-3) and 2-ethyl-hexyl-4-methoxycinnamate (octinoxate, EHMC) are one of the major compounds of sunscreens for protect skin or products from UV radiation. BP-3 and EHMC are known endocrine disrupting chemical (EDC) and have been shown to cause endocrine disorders in a variety of organisms. In previous study, the effect of BP-3 (0.5, 5, 50 and 500 ng mL-1) and/or EHMC (0.5, 5, 50 and 500 ng mL-1) were examined in the maturing oocytes of longchin goby (Chaenogobius annularis); showed a significant increase of estradiol (E2) level in the BP-3, 5 ng mL-1. Therefore, in this study, we investigated the effects of mixture (BP-3 and EHMC) on estrogens (estrone, E1; E2) levels and GVBD using maturing oocytes of longchin goby. We performed two separate similar experiments. The oocytes with average diameters of 730, 770 and 800 ㎛ were incubated with different concentrations (BP-3, 5 ng mL-1 with EHMC, 0, 5, 50, and 500 ng mL-1) for 24 hours under 15 ℃ and 20 ℃ water temperature (WT). GVBD was observed under a microscope, E1 and E2 levels were measured by radioimmunoassay (RIA). GVBD induction was significantly increased in 15 ℃, 770 ㎛, BP-3, 5 ng + EHMC, 50 ng mL-1 compared to the control. E1 level was significantly increased in 20 ℃, 770 ㎛, BP-3, 5 ng mL-1 and E2 level was increased in 15 ℃, 770 ㎛, BP-3, 5 ng mL-1 compared to the control.