저질의 높이 변화에 따른 미꾸리, Misgurnus Anguillicaudatus의 혈액학적 성상, 항산화 및 생리학적 변화

Abstract

In order to confirm changes in hematological, antioxidant and psychological stress response of pond loach following exposure to different muddy sediment height each water tank, centimeter levels of 0, 3, 6, 9, 12 cm for 4 weeks were exposed pond loach, misgurnus anguillicaudauts(length 8.75±1.75cm, weight 10.52±1.62g). survival rate significantly increase above 6 cm height of sediment. Growth factor not show significantly changed. All water tank environment factor, ammonia, nitrate, phosphate, COD of sediment did not show significantly changed. Hematological marker of RBC count, Hemoglobin, Hematocrits did not showed a significantly changed. Plasma organic component glucose decreased at 9 cm at 2 weeks, and at 6 cm at 4 weeks, but total protein did not show significant changed. Plasma enzymes, GOT and GPT, show significant decrease at 9 cm at 4 weeks. Plasma minerals calcium and magnesium did not show significant changes. Antioxidant response was analyzed using liver, gills and intestine. SOD observed significant decrease at liver and gill tissue. but did not show observed changed at intestine. stress hormone Cortisol show decrease above 3 cm at 4 week. HSP70(Heat shock protein 70) observed significant decrease liver, gill and intestine. In conclusion, muddy sediment induce to relive stress to pond loach, misgurnus angullicadatus.