폐해조류 Sargassum sp.로부터 CRISPR/Cas9 재조합 균주를 이용한 D-lactic acid 생산

Abstract

Sargassum sp. is influxed to the Jeju coast in large quantities every year. This not only harms the aquaculture farms, but also spoils the scenery of the beach and generates odors. The third-generation biomass, algae, does not cause agflation and is attracting attention as a carbon-neutral energy resource. In this study, waste seaweed Sargassum sp. is used as feedstock to produce D-lactic acid. Thermal acid pretreatment conditions (H2SO4, concentration, reaction time, biomass concentration) were optimized using response surface methodology(RSM). The condition of 10% of Sargassum sp. After adding 450 mM sulfuric acid at 121 °C for 120 min was selected as the optimal thermal acid hydrolysis condition. When enzymatic saccharification was performed for 24 hours with a 1:1 mixed enzyme of Cellic® CTec2:HTec2 after thermal acid hydrolysis, a maximum of 29.32 g/L of reducing sugar was obtained. D-lactic acid was produced using a S. cerevisiae strain engineered via CRSPR/Cas9 using reducing sugar obtained from Sargassum sp.