항산화 활성 황다랑어(Thunnus albacare) 근육 가수분해물의 동정 및 응용

Abstract

Fish consumption is increasing worldwide, and the consumption of preserved foods such as tuna cans is rapidly increasing due to COVID-19 pandemic, resulting in a significant amount of tuna waste. Efficient treatment means to solve environmental problems caused by such wastes are required. Fish waste is rich in protein and is a valuable raw material for reutilization. In this study, as a means of reutilization yellowfin tuna waste, antioxidant activity peptides were extracted from tuna hydrolysate and plants with high antioxidant activity were cultivated. Among several hydrolysis methods, enzymatic hydrolysis produced tuna hydrolysate with the highest antioxidant activity with 93.75% (DPPH radical scavenging activity). Antioxidant peptide fraction (< 2 kDa) from which impurities were removed by solvent extraction and ultrafiltration was separated. Then, the active antioxidant fraction was applied to an Ultra High Resolution Q-TOF LC MS/MS system, followed by MS/MS analysis. The antioxidant peptide had linear structure consisting of amino acids with sequence NH-GLLGAP-COOH. Since antioxidant activity helps improve plant growth, it was used for hydroponic cultivation of wheat sprouts as liquid fertilizer. As a result of hydroponic cultivation for 7 days, wheat sprout growth was improved by 9.89% in the fraction with the highest antioxidant activity (< 2 kDa) compared to the control (DW). Therefore, tuna hydrolysate is a useful resource for the production of antioxidant peptides and has a plant growth-promoting effect, which is valuable in that it can be applied as a natural liquid fertilizer.