전복내장으로부터 분리한 단백질분해효소 생산 균주의 분리동정 및 어류 단백질 분해 특성

Abstract

The gastrointestinal tracts of aquatic animals provide a unique environment for microbial growth. Various microbial communities in the gut produce enzymes to adapt these conditions. This study aimed to isolate and select bacteria from the gut of abalone (Haliotis discus hannai) for producing protease. The bacteria with high protease activity were isolated from the abalone intestine. Selected bacteria was identified by 16S rRNA sequence analysis, and investigated their biochemical characteristics using API 50 CHE kit. The optimal culture conditions to maximize protease production were established using response surface methodology (RSM). Total 15 bacteria were isolated from the gut of abalone, among which six bacteria with protease activity were confirmed. Among them, T14 showed the highest protease activity as 2.42 ± 0.19 unit/mL and was finally selected for the protease-production. T14 was displayed 99.71% similarity to Vibro fluvialis NBRC 10315. The optimal culture conditions were determined to be pH 7.85, temperature of 29.49℃, and a NaCl concentration of 2.69% through RSM modeling. The predicted maximum protease activity was 3.20 unit/mL. As a result of verification, actual protease activity was 3.19 ± 0.12 unit/mL. The application results of the T14 strain to fish by-product revealed lower protease activity compared to commercial proteases. However, it exhibited the potential for protein degradation by partially hydrolyzing the major proteins present in fish by-product. This study suggests the applicability of protease-producing bacteria isolated from abalone intestine to produce protrase enzyme.