2004년 베링해에서 채집된 연어 (Oncorhynchus keta)의 유전적 조성에 관한 연구
- Alternative Title
- Genetic stock identification of chum salmon (Oncorhynchus keta) collected from the western Bering Sea during summer-autumn 2004
- Abstract
- Population structure of chum salmon (Oncorhynchus keta) was examined using mtDNA of 3,266 specimens collected from 76 populations along the Pacific rim. Both phylogenetic tree and AMOVA test indicated that the entire populations can be re-grouped as four regional groups (Korea-Japan-Primorie, Russia, Northwest Alaska, and other North America). Also, in order to determine the regional proportions of ocean mixtures, I examined mtDNA of chum salmon collected from the western Bering Sea during Sept. 26 ∼ Oct. 23, 2004. The Statistics Program for Analyzing Mixtures (SPAM) estimation was performed for regional stock contribution using maximum likelihood algorithm. In fall 2004, stock composition in the wetern Bering Sea was 42% Korea-Japan-Primorie, 41% Russian, 13% Northwest Alaskan, and 4% other North American groups.
- Issued Date
- 2007
- Awarded Date
- 2007. 2
- Type
- Dissertation
- Publisher
- 부경대학교 대학원
- Alternative Author(s)
- Kang, Min-Ho
- Affiliation
- 부경대학교 대학원
- Department
- 대학원 해양생물학과
- Advisor
- 김수암
- Table Of Contents
- INTRODUCTION = 1
1. Distribution of chum salmon in the North Pacific = 1
2. DNA marker technologies in stock identification for chum salmon = 4
3. Objectives = 5
MATERIALS AND METHODS = 6
1. Re-analyzed baseline data = 6
1.1 Sampling locations for baseline data = 6
1.2 Analytical method for baseline data = 6
2. Mixed-stock data = 8
2.1 Fish samples = 8
2.2 DNA extraction and PCR amplification = 11
2.3 Direct sequencing of PCR products = 12
2.4 Analytical method for mixed-stock data = 13
RESULTS = 14
1. Distribution of mtDNA haplotypes in chum salmon along the Pacific-rim = 14
2. Geographic differentiation in the Pacific-rim populations = 17
3. Haplotype composition and distribution of chum salmon in the western Bering Sea = 21
4. Stock composition of mixtures in the western Bering Sea = 24
DISCUSSION = 27
1. Population structuring of chum salmon in the North Pacific = 27
2. Genetic Stock Identification (GSI) of mixtures in the North Pacific = 28
REFERENCES = 31
- Degree
- Master
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