굴 가수분해물의 기능 특성 및 식품에의 응용

Abstract

The objectives of the experiment were to determine the optimum reaction for the production of the functional oyster hydrolysates by enzymatic and acidic hydrolysis. The optimal reaction conditions of the enzymatic hydrolysis were in 1% TGase for 60 min at 30℃ for TGase cross - linking, 1% protamex for 60 min at 40℃ for 1st hydrolysis, 1% neutrase for 60 min at 55℃for 2nd hydrolysis, respectively. The reaction mixture was heated at 100℃ for 60 min to inactivate. The acid hydrolysis was done for 22 hr at 95℃ in 10 N HCl. The degree of hydrolysis(%) of acid hydrolysates was affected by the concentration of HCl and the time of hydrolysis, while temperature had little effect on the DH(%). We have investigated the properties of the functional peptides from oyster hydrolysates. The peptides were determined antiradical activity EC50 by DPPH. The EC50 of TGase-P-N, P-N, and acidic hydrolysates were 3.26, 4.86, and 0.63 mg/mL for lyophilized powder; 1.88, 2.04, and 0.56 mg/mL for ionic exchanger chromatography; and 0.08, 0.19, and 0.05 mg/mL for size exclusion chromatography, respectively. Antioxidant against oxidation of linoleic acid were 0.046, 0.050, and 0.104 unit for lyophilized power; 0.074, 0.077, and 0.072 unit for ionic exchanger chromatography; and 0.140, 0.398, and 0.111 unit for size exclusion chromatography, respectively. Xanthine oxidase activity were 61475, 46106, and 135245 units/mg-protein for lyophilized powder; 8702, 8197, and 9221 units/mg-protein for ionic exchanger chromatography; and 12039, 5379, and 5635 units/mg-protein for size exclusion chromatography, respectively. Ferricyanide reducing power was 0.394, 0.407, 0.911 unit, for lyophilized powder and Ion-exchanger; 0.431, 0.388, 0.205 unit, for ion-exchanger chromatography and Size exclusion; 0.510, 0.125, 0.261 unit, for size exclusion chromatography, respectively. ACE inhibition were 38.1. 48.9, and 85.1 unit for lyophilized powder; 86.7, 87.0, and 33.8 unit for ionic exchanger chromatography; and 80.0, 79.8, and 73.5 unit size exclusion chromatography, respectively.