넙치, Paralichthys olivaceus의 초기 생활사에 미치는 중금속의 영향

Abstract

This study was conducted to investigate effects on the early life stage of the fertilized egg, embryo and larvae of olive flounder, Paralichthys olivaceus exposed copper and chrome for 40hour in egg and 60hour in embryo-larvae and 20hour in post-larvae. Copper and chrome concentration were 10, 20, 40 and 80 ㎍ L^(-1); 50, 100, 500, 1000 and 2000 ㎍ L^(-1), respectively. Fertilized egg stages, the survival rate, hatching rate and malformation rate was investigated. For embryo-larvae and post-larvae stages, the survival rate and malformation rate were only investigated, in this test. Short term toxicity about hatching rate, the survival rate and the malformation rate of olive flounder, P. olivaceus fertilized egg and survival rate, malformation rate of P. olivaceus embryo-larvae and post-larvae by copper and chrome exposure. After fertilized egg that expose copper and chrome begins an experiment, the survival rate looked dwindling tendency before end. In the case of control group, showed the survival rate of average 86.38% to experiment end, but significantly decreased in 20, 40 and 80 ㎍ L^(-1) in the case of copper exposure. Fertilized egg that expose to chrome significantly decreased in 500, 1000 and 2000 ㎍ L^(-1) than control group. LC_(50) was the copper 44.27 ㎍ L^(-1) and the chrome 1318.73 ㎍ L^(-1) in about survival rate of the fertilized egg. In the case of control group, showed hatching rate of average 89.02% to experiment end, but significantly decreased in 10, 20, 40 and 80 ㎍ L^(-1) concentration in copper exposure. In the case of chrome exposure, significantly decreased in all exposure. LC_(50) was the copper 33.95 ㎍ L^(-1) and the chrome 905.35 ㎍ L^(-1) in about hatching rate of the fertilized egg. Significantly increased in all concentration in copper exposure than control group (5.29%) in the malformation rate of fertilized egg that expose copper and chrome. Significantly increased in all concentration in group's case which expose chrome. EC_(50) was the copper 37.58 ㎍ L^(-1) and the chrome 955.12 ㎍ L^(-1) in about malformation rate of the fertilized egg. After embryo-larvae that expose copper and chrome begins an experiment, the survival rate looked dwindling tendency before experiment end. Showed the survival rate of 89.81% to experiment end in case of group's control group that expose copper, but significantly decreased in 10, 20, 40 and 80 ㎍ L^(-1) concentration in the case of copper exposure. Also, significantly decreased in all concentration to experiment end in group's case which expose chrome. LC_(50) was the copper 37.12 ㎍ L^(-1) and the chrome 1129.74 ㎍ L^(-1) in about survival rate of the embryo-larvae. Showed the malformation rate of 7.62% to experiment end in case of control group that expose copper in embryo-larvae the malformation rate that expose copper and chrome, but displayed significantly increase in all concentration in the case of exposure. Significantly increased in all concentration in group's case which expose chrome. EC_(50) was the copper 16.79 ㎍ L^(-1) and the chrome 684.48 ㎍ L^(-1) in about malformation rate of the embryo-larvae. After post-larvae that expose copper and chrome begins an experiment, the survival rate looked dwindling tendency before end. Showed the survival rate of 99.34% to experiment end in case of group's control that expose copper, but significantly decreased in all concentration in the case of exposure, and significantly decreased in all concentration in case of group's that expose chrome. LC_(50) was the copper 34.81 ㎍ L^(-1) and the chrome 980.64 ㎍ L^(-1) in about survival rate of the post-larvae. Showed the malformation rate of 8.91% to experiment end in case of group's control that expose copper in post-larvae's the malformation rate that expose copper and chrome, but displayed significantly increase in all concentration in the case of exposure, and exposure significantly increased in all concentration in chrome exposure group. EC_(50) was the copper 36.25 ㎍ L^(-1) and the chrome 933.59 ㎍ L^(-1) in about malformation rate of the post-larvae.