초임계 유체를 이용한 게 껍질로부터 기능성 물질의 회수

Abstract

The solubility of astaxanthin in carbon dioxide were measured under the supercritical conditions of a pressure range from 80 to 300 bar, and temperature range from 303 to 333 K, using a dynamic flow-type. The solubility of astaxanthin increasing from 0.78 × 10-5 to 4.89 × 10-5 with higher temperature and pressure maintains certain density of supercritical carbon dioxide(SC-CO2). The solubility data obtained were applied to the Chrastil model, based on the density of carbon dioxide. The data fitted well with the Chrastil model at most experimental conditions. According to solubility measurement result, astaxanthin extracted from crab shell by SC-CO2 at 308, 313, 318 K and 100, 200 bar. TBARS(Thiobarbituric Acid Reactive Substances) value in extract compared other anti-oxidant that α-tocopherol and ascorbic acid. The effect of operating parameters(reaction temperature and time) on the behavior of chito-oligosaccharide production from hydrothermal decomposition of crab shell was investigated. High temperature and high pressure water hydrolysis of chitin and chitosan were carried out 423 K to 523 K and 1 to 3 hour. The TLC analysis result of chitin hydrolysis show high recovery at 1 hour, 473 K and in case of chitosan show at 1 hour, 523 K. It was found that SC-CO2 extraction and sub-critical hydrolysis reaction can be used as an alternative separation technology to recover functional material from crab shell.