꽈배기모자반 추출물의 항산화능에 미치는 열, pH 및 감마선 조사의 영향

Abstract

This study was designed to evaluate the antioxidant abilities of the extracts from Sargassum siliquastrum treated with heat, pH and γ-irradiation. The changes in antioxidant ability were estimated using total phenolic compounds, TBARS(thiobarbituric acid reactive substances), DPPH(1,1-diphenyl-2-picrylhydrazyl) radical scavenging effect, chelating effect and reducing power. In the results, the antioxidant abilities of the ethanol extract was higher than that of the water extract in TBARS and DPPH radical scavenging effect. The mechanism of inhibitory effects by which the extracts protect against a lipid peroxidation may involve radical scavenging capability. In addition, the stability of the ethanol extract to temperature (60℃ to 100℃) and pH (2 to 10) treatment was investigated. At the thermal stability test to the ethanol extract was proven to maintain a good antioxidative activity. The ethanol extracts treated with pH 4 to 10 didn't show any significant change (p≤0.05) in their antioxidant ability. But the ethanol extract treated with pH 2 indicated higher than the others. The ethanol extract and powder were irradiated with Co^(60) γ-ray in the dose range of 5 to 100 kGy, and the water extract also was irradiated(3 to 20 kGy). The irradiated extracts were analyzed to investigate the antioxidative activities, including total phenolic compounds, radical scavenging effect and TBARS, and the physical properties such as viscosity and color. Gamma irradiation decreased slightly total phenolic compounds of the irradiated ethanol extracts, but DPPH radical scavenging effects and TBARS didn't show any significant change (p≤0.05). The irradiated ethanol extracts showed higher superoxide radical and hydroxyl radical scavenging effects than the non-irradiated. The ethanol extracts from irradiated powders didn't indicate any significant change (p≤0.05) in DPPH radical scavenging effects. In the irradiated water extracts, total phenolic compounds and DPPH radical scavenging effects were improved. In the physical properties, viscosity and color of the irradiated extracts were lower than that of the non-irradiated. In conclusions, Sargassum siliquastrum was a potential source of antioxidant component and was stable at heat and pH treatement. Moreover, γ-irradiation improved antioxidant abilities and physical properties of Sargassum siliquastrum.