원적외선 활성수가 조피볼락 (Sebastes schlegeli)의 자연발생된 지방간 예에 미치는 병리조직학적 영향

Abstract

본 연구는 고등동물에서 생체활성을 통해 건강개선 효과를 나타내는 원적외선 활성수(이하 기능수)가 어류에 미치는 영향을 과학적으로 증명하고자 실시하였다. 어류에서는 비특이적 방어 체계가 주를 이루는데, 이는 어류의 기본건강과 연관하여 그 효력이 강해지거나 약해진다. 본 연구에서는 기능수가 어류생체에 미치는 영향을 알아보기 위하여, 자연발생된 지방간증을 보이는 양식 조피볼락(Sebastes schlegeli)을 질병모델로 하여 기능수에서의 사육시 나타나는 영향을 집중 조명하였다. 기능수를 사육수로하여 5주 동안 조피볼락을 사육하며 임상증상을 관찰하였다. 실험기간 중 1주, 3주, 5주차에 실험어를 부검하여 간장을 중점적으로 육안적 및 병리조직학적 변화를 관찰하였다. 실험결과, 기능수의 적용을 통해 어류의 지방간증이 정상에 가깝게 개선되었으며, 아가미 및 심장과 같은 기타장기의 상태개선과 임상적으로 긍정적인 영향을 미치는 것으로 나타났다. 이상의 결과를 통해 원적외선 활성수가 어류간장의 지방간증을 개선하고, 어체를 건강하게 만드는 생리활성 작용을 한다는 결론을 얻었다.

As part of research to get scientific background for biological effect of far infrared ray activated water, a histopathological study was performed, using a group of cultured rockfish with naturally occurred fatty liver. DM bio functional seawater of a company, Daemyung Bio, was applied as far infrared ray activated water. By rearing the diseased fish with the functional seawater, the biological effect on the fatty liver was histopathologically evaluated one, three and five weeks after rearing. Naturally occurred fatty liver of rockfish grossly showed severely mottled appearance and microscopically severe vacuolation with large, cytoplasmic fat globules in HE stain. Each of hepatocytes had nuclei marginally dislocated. Sinusoidal capillaries and Disse space were barely recognizable. As early as from one week after rearing, most of livers showed more even coloration on their surface and reduction of cytoplasmic fat globules in number and size. 5 weeks after, most of fish became to microscopically have hepatocytes very close to those of normal in morphology. The number and size of fat globules were markedly reduced. Sinusoidal area including Disse space was almost completely restored. Nuclei of hepatocytes returned to the center which was normal. Hyaline inclusions suggesting secondary lysosomes were nearly not recognizable in most of individuals one week after. Restoration of hepatocytes was consistently accompanied by infiltration of glycogen. On the other hand, all individuals of conventional sea water group used as control still had findings of fatty liver both grossly and microscopically even 5 weeks after. From these results it is suggested that far infrared ray activated seawater apparently facilitates the recovery process toward normal from naturally occurred fatty liver of rockfish.