Histopathological Health Evaluation of Cultured

Abstract

A histopathological health evaluation study for olive flounder, Paralicthyes olivaceus, with different size based on total body length and body weight was carried out. Two groups of fishes were administered for the study from the same culture system and age group-the big sized fishes (fast growing) and the small sized fishes (slow growing) in terms of total length and body weight. Samplings were done two times and six fishes were taken for each group in each sampling. For sampling I, total length and body weight were 27.58±2.10cm and 221.83±23.72g for big sized fishes (fast growing) and 22.58±0.58cm and 125.83±11.51g for small sized fishes (slow growing) respectively. In case of sampling II, total length and body weight were 26.70±1.64cm and 404.00±58.26g for big sized fishes(fast growing) and 22.03±0.82cm and 215.00±38.89g for small sized fishes (slow growing) respectively. Clinical record, blood chemistry; total protein (Tp), hemoglobin (Hb), hematocrit (Ht), functional indices (HSI and HHI) and histological study of different organ (liver, stomach, intestine, spleen, kidney and heart) were done for each individual of each group for the experiment. Moderate to severe fatty change of liver were observed in both groups of fishes in case of sampling I. Moderate to severe atrophy of liver were clearly observed in both groups in sampling II. Focal epicarditis were observed in sampling I for big sized fishes and vacuolative changes of gastric gland of big sized fishes were clearly observed in sampling II under light microscopic examination. Tp, Ht, and HHI were recorded significantly higher for big sized fish group in sampling I. In case of sampling II only HHI was recorded significantly higher in big sized fish group. So it can be concluded fast growing individuals are more vulnerable to disease in terms of histopathological evaluation.