강도다리 (Platichthys stellatus) 방류산과 자연산의 형태 및 분자 변이 비교

Abstract

Starry flounder, Platichthys stellatus, family Pleuronectidae is benthic species and distributed in Northern middle East Sea through all over the North Pacific. Starry flounder has a royal reputation for raw fish not only in Korea but also China than olive flounder. Even though demand is increasing, supply does not reach it due to the reduction of resources and closing season. Thus over 500,000 seeds has been releasing for recover since 2006. However, used individual for producing artificial seeds should be considered how they effect wild environment due to their limited gene pool. Therefore, this research is about comparing two groups in morphology, genetic diversity of mtDNA control region and MS DNA. The result of morphological modification analyzation for each 30 released and wild individuals from Ul-jin and Po-hang, East Sea of Korea between 2009 December and 2010 November showed distinct blackening in blind side and deformation in anal fin ray. According to mtDNA control region analysis result, genetic distance in released group was 0％∼1.4％, wild group was 0％∼1.1％ and between two groups were 0％∼1.4％. There were no significant differences in intra and intergroup. Two groups mixed well in NJ-tree. There was no significant differences in intergroup genetic diversity as well as low FST(-0.00849) value(p>0.05). Size of msDNA alleles analyzed by 6 markers ranged 188­266bp. Total number of alleles(AT) were 5.5 in released group and 7 in wild group. Number of allelic richness(AR) ranged 5.0-8.0, wild group (7.0) was 21.43% lower than released (5.5) group. Observed heterozygosity(HO) and expected heterozygosity(HE) for released group 0.550 and 0.592 were lower than wild group 0.700 and 0.737. Average inbreeding coefficient(FIS) was higher in released group; released group was 0.073 and wild group was 0.045. FST, degree of differences in genetic diversity showed significant differences between two groups (0.0208). In conclusion, morphology and msDNA differences between two groups were distinct contrast to mtCR. Therefore, msDNA will be suitable for understanding of genetic information through ecological view.