모자반 및 비틀대 모자반 에탄올 추출물의 항염증 효과

Abstract

Inflammation is a beneficial host response to external stimuli or cellular damage that leads to the release of a complex array of inflammatory mediators, finalizing the restoration of the tissue structure and function. However, prolonged inflammation contributes to the pathogenesis of various inflammatory diseases. The macrophage is a potent cell which plays a critical role in the inflammatory reaction and influences between innate and adaptive immunity. It produces many kinds of mediators including nitric oxide(NO), prostaglandins, pro-inflammatory and cytotoxic cytokines such as interlerkin-1 beta(IL-1β), IL-6 and tumor necrosis factor-alpha(TNF-α). Currently, a strong interest in developing new anti-inflammatory drugs from various natural products has been growing. It may inhibit or prevent a variety of inflammatory diseases by suppressing the secretion of these inflammatory mediators in activated macrophages. Therefore, the anti-inflammatory effects of Sargassum fulvellum(SFEE) and Sargassum sagamianum(SSEE) ethanol extracts were investigated using LPS-induced inflammatory response in this study. To examine the potential anti-inflammatory properties of SFEE and SSEE, the cell proliferation, NO, TNF-α, IL-6 and IL-1β were measured. The expression of COX-2 was also assessed by immunoblotting to evaluate the inhibitory actions of SFEE and SSEE on prostaglandins. As a result, there were no cytotoxicity in the macrophage proliferation treated with SFEE and SSEE compared to the control. NO levels decreased with increasing concentration of SFEE and SSEE and was inhibited up to 50%. Moreover, the secretion of TNF-α, IL-1β and IL-6 were suppressed in a dose-dependent manner, and IL-6 and IL-1β inhibition activities were over 50% at 0.1 μg/mL. The expression of COX-2 was also inhibited by SFEE and SSEE which demonstrate strong anti-inflammatory effect through its ability to reduce prostaglandin. These results suggested that SFEE and SSEE may have significant effects on inflammatory factors and be a potential anti-inflammatory therapeutic materials.