절임배추 생산 중의 위해요소
- Alternative Title
- Minimization of Hazard Management and Process Standization
- Abstract
- This research aimed to investigate the quality of conventional salted cabbages. Here, we studied the general characteristics and microbiological aspects of purchased salted cabbage samples from 15 companies. The purchased salted cabbages used a sun-dried salt, but two samples used a mixture of sun-dried salt and processed salt. There were 4 times of washing, 3 steps of washing after automatic washing, and 3 steps of washing after bubble washing as washing methods for the salted cabbages and ground water was used as the washing water. Also, three samples received Hazard Analysis and Critical Control Points (HACCP) certification. The salt contained in the purchased salted cabbages ranged from 0.5% to 2.0%, representing low salted cabbages. The pH of the purchased salted cabbages ranged from 5.32 to 6.47, and hardness ranged from 1,997 g to 3,665 g. Rewashing was necessary before using some salted cabbages due to foreign materials such as insects, soil, etc. Total bacterial counts of the purchased salted cabbages ranged from 3.36 log to 6.06 log and coliform bacteria ranged from below 1 log to 6.05 log, whereas other pathogens were not detected. Salted cabbage products purchased from different companies at 4 different districts in South Korea were detected in this study. Cabbage and salt are the main materials for salted cabbage manufacture. The results of general bacteria contaminated in the samples were 1.4×105, 6.4×105, 1.7×107, 3.6×107 CFU/g in cabbage and 2.7×103 CFU/g in salt, respectively. The results of coliforms were detected as 2.4×104 CFU/g, and there was no Escherichia coli in any sample. Staphylococcus aureus was detected in cabbage as 9.9×102, 8.0×101, and 3.0×103 CFU/g, Bacillus cereus was also found in cabbage as 4.1×103 and 1.0×101 CFU/g. Total bacterial counts ranged from 1.4×101∼4.4×105 CFU/g were detected in salting solution, from 1.5×104∼1.2×108 CFU/g in dehydrated salted-cabbage, from 9.4×104~1.3×108 CFU/g in minced salted-cabbage. The results of E. coli in samples from different companies were different from one to another. The results of the contamination of S. aureus and B. cereus showed positive in salting solution and dehydrated salted-cabbage at a portion of companies. Vibrio paraheamolyticus was detected in salting solution. During the manufacture processing of Kimchi, microorganisms were detected in cabbages salted in different concentrations of salt solution at 8%, 10%, 12% and 15% for 5 ∼ 20 hours. As the results, 3.5×105∼1.7×106 CFU/g, 3.4×105∼2.5×106 CFU/g, 5.4×105∼2.3×106 CFU/g, 4.0×105∼2.3×106 CFU/g were detected for E. coli in samples at different treatment conditions. 1.9∼4.1×104 CFU/g, 4.1×103∼2.8×104 CFU/g, 1.5∼7.9×103 CFU/g, 2.2∼6.7×104 CFU/g were detected for S. aureus in samples at different treatment conditions. Salmonella typhimurium was detected in salted cabbage with various salt concentration after salting for 5 hrs, the result ranged from 2.5×105 to 3.9×106 CFU/g, and change of microorganism was the smallest in salted cabbage under the concentration of salting solution at 10% for 15hours. The cabbage salted in 10% salting solution for 15 hours were washed with water for 2 and 3 times, with chlorine for 3 times, and with acetic acid for 3 times. E. coli was detected in the samples washed with water for 2 and 3 times, washed with chlorine for 3 times. The contamination of S. aureus was 3.1×105 CFU/g in the samples washed with water for 2 times, 5.7×103 CFU/g in the samples washed with acetic acid for 3times, 3.6×105 CFU/g in the samples washed with water for 3 times and same amount in the samples washed with chlorine for 3 times. According to the results, the contamination of S. aureus was 5.6×103 CFU/g lower in samples washed with chlorine and acetic acid than that in samples washed with water. In case of S. typhimurium, it has been detected in samples washed with water and chlorine, 3.0×101 CFU/g as the lowest concentration among all the samples was measured in the samples washed with acetic acid for 3 times. This research aimed to investigate the quality of conventional salted cabbages. Here, we studied the general characteristics and microbiological aspects of purchased salted cabbage samples from 15 companies. The purchased salted cabbages used a sun-dried salt, but two samples used a mixture of sun-dried salt and processed salt. There were 4 times of washing, 3 steps of washing after automatic washing, and 3 steps of washing after bubble washing as washing methods for the salted cabbages and ground water was used as the washing water. Also, three samples received HACCP certification. The salt contained in the purchased salted cabbages ranged from 0.5% to 2.0%, representing low salted cabbages. The pH of the purchased salted cabbages ranged from 5.32 to 6.47, and hardness ranged from 1,997 g to 3,665 Re-washing was necessary before using some salted cabbages due to foreign materials such as insects, soil, etc. Total bacterial counts of the purchased salted cabbages ranged from 3.36 log to 6.06 log and Coliform bacteria ranged from below 1 log to 6.05 log, whereas other pathogens were not detected.
- Author(s)
- 김진희
- Issued Date
- 2012
- Awarded Date
- 2012. 2
- Type
- Dissertation
- Keyword
- 절임배추
- Publisher
- 부경대학교
- URI
- https://repository.pknu.ac.kr:8443/handle/2021.oak/9138
http://pknu.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000001965920
- Alternative Author(s)
- Jin Hee Kim
- Affiliation
- 부경대학교 일반대학원 식품공학과
- Department
- 대학원 식품공학과
- Advisor
- 양지영 교수님
- Table Of Contents
- Abstract
List of Tables
List of Figures
Ⅰ. 서 론 1
Ⅱ. 재료 및 방법 7
1. 시료채취 7
2. 시료 전처리 7
3. 배지 및 시약 8
4. 균주배양 8
5. 병원성 및 위생지표 세균 8
6. 미생물 분석 9
6.1 Total aerobic bacteria count 9
6.2 Colifrom group, Escherichia coli 9
6.3 Staphylococcus aureus 10
6.4 Salmonella typhimurium 10
6.5 E. coli O157:H7 11
6.6 Listeria monocytogenes 12
6.7 Bacillus cereus 12
6.8 Yersinia enterocolitica 13
6.9 Vibrio parahaemolyticus 14
6.10 Clostridium perfringens 15
6.11 Campylobacter jejuni 16
6.12 공중낙하균 16
6.13 PCR을 이용한 식중독균 검출법 17
7. 일반성분 분석 19
7.1 염도 분석 19
7.2 산도 분석 19
7.3 pH 분석 19
7.4 Rheometer에 의한 절임배추 Texture 분석 19
8. 보존료 분석 20
9. 타르색소 분석 25
10. 중금속 분석 25
11. 잔류농약 분석 26
12. 물리적 분석 29
Ⅲ. 결과 및 고찰 30
Part 1. 안전한 절임배추 생산을 위한 위해요소 저감화방안 30
1. 시판 절임배추의 품질 및 안전성 평가 30
1.1 조사방법 30
1.2 시판 절임배추의 위생변화 30
1.3 시판 절임배추의 품질변화 33
1.4 시판 절임배추의 병원미생물변화 33
2. 절임배추의 생산 공정별 위해요소분석 38
2.1 시료 38
2.2 원재료의 배추·소금에 대한 위해미생물 분석 38
2.3 절임배추 제조 전·후 중금속 분석 41
2.4 절임배추의 생산 공정 중의 미생물 오염도 변화 41
2.5 시판절임배추의 생산 시 주요공정 생산 관리점 파악 47
3. 절임배추의 생산 공정의 주요 위해요소 제어방안 47
3.1 절임공정 시간 및 염수절임 구간설정 변화 47
3.2 절임공정 조건에 따른 위해 미생물 변화 48
3.3 세척방법에 따른 위해 미생물의 변화 54
Part 2. 안전관리 절임배추의 HACCP 매뉴얼 59
Ⅳ. 요 약 133
Ⅴ. 참고문헌 135
- Degree
- Doctor
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