조피볼락, Sebastes schlegeli의 생리ㆍ생화학적 변동에 미치는

Abstract

Ⅴ. 요 약

구리(Cu)는 모든 생물에게 필요한 미량원소이지만, 중금속에 속하기 때문에 비록 미량일지라도 수중생물에 농축, 축적이 가능하며, 특히 어류에 급ㆍ만성 독성을 일으키고 장ㆍ단기적으로 해양생태계에 여러 가지 영향을 미칠 수 있고, 어체의 혈액성상, 성장, 행동, 효소활동, 축적 등을 일으킬 수 있고 먹이연쇄를 통해 인체에까지 영향을 미치므로 수생물의 오염은 인류의 공중보건에도 직결되는 심각한 문제가 될 수도 있다. 혈액성상의 변화는 Ht (Heamatocrit), RBC (Red Blood Cells) count, Hb (hemoglobin)을 조사하였다. Ht, RBC count의 경우 18℃, 23℃, 28℃의 경우 control, 100ppm, 200ppm의 모든 구간에서 대체적으로 감소하는 경향이 나타났으며, Hb의 경우, 18℃, 23℃, 28℃에서 control, 100ppm, 200ppm의 모든 구간에서 대체적으로 증가하는 경향이 관찰되었다. 혈액 무기성분 중 혈청 내 칼슘 (calcium), 마그네슘 (magnesium)의 농도는 18℃, 23℃, 28℃의 100ppm, 200ppm에서 모두 유의적 증가를 나타냈다. 총 단백질 (total protein)의 경우 18℃, 23℃, 28℃에서 Control과 비교했을 때 100ppm, 200ppm 모든 농도 구간에서 유의적 증가를 나타냈고, 글루코즈(glucose)도 총 단백질과 마찬가지로 18℃, 23℃, 28℃ 100ppm, 200ppm 모든 농도 구간에서 유의적 증가를 나타냈다. 혈청효소성분인 GOT, GPT, LDH는 모든 구간에서 모두 유의적인 증가가 나타났다. 혈장 콜티졸과 항산화 작용을 하는 GSH, GST는 간, 아가미, 신장에서 모두 증가하였고, GPx의 활성은 간, 아가미, 신장에서 모두 감소하였다. GR은 간과 신장에서는 모든 구간에서 유의적 증가를 나타냈으나 신장의 23℃ 100ppm, 200ppm 농도 구간에서는 유의성이 관찰되지 않았다.

Combined effect of Cu and temperature on physiological and biochemical change of rock fish, Sebastes schlegeli

Su Kyong Baeck

Department of Fish Pathology, Graduate School, Pukyong National University

Abstract

Copper (Cu) is an essential metal for all organisms including fish. It has an important role in metabolism and its concentration is well regulated. However, Cu is one of the most toxic metals to fish and affects various blood parameters, growth, behavior, enzyme activity, and reproduction. Exposed to this toxicant, fish is under stress, and consequently, it can also affect ability to maintain homeostasis. This study was conducted to investigate effects on hematological parmeters and oxidative stress and concentration of cortisol in rock fish, Sebastes schlegeli exposed copper for 4 days. Temperature condition was 18℃, 23℃ and 28℃, copper concentration was 100ppm, 200ppm, respectively. The RBC count and Ht were significantly decreased in exposed rock fish compared to control group. The rock fish exposed to all copper concentration started to increase significantly in serum calcium. Serum magnesium started to increase significantly

to all copper concentration but a little decreased at 23℃. Although serum total protein concentrations was significantly increased compared to the control group at copper concentration 100ppm, serum glucose concentration was found to be significantly increased over the control group. A significant increment of GOT, GPT and LDH activities in the rock fish serum was noticed at all temperature conditions. GSH and GST activities in the rock fish serum was increased in liver, gill and kidney, GPx activities was decreased. GR activities was increased in liver and gill however, significance was confirmed only in kidney. In Summary, copper can lead to stress to fish, homeostasis was wrecked in metabolic processes.