PUKYONG

타가영양성 Chlorella 종의 탐색 및 최적 배양 조건

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Alternative Title
The screening and optimum culture condition of heterotrophic Chlorella species
Abstract
Many phototrophic Chlorella species are useful for high value products such as CGF (Chlorella Growth Factor) and carotenoids. However, the growth of phototrophic Chlorella is often slow due to light limitation. Some phototrophic Chlorella can also grow heterotrophically with organic substrate in dark condition. Heterotrophic culture is easy to control and achieve to the fast growth and high yield of valuable products in mass culture. However, heterotrophic Chlorella species are limited.
In this study, thirty-three Chlorella species were tested on the growth in heterotrophic condition and three species from freshwater were found as heterotrophic Chlorella growing well with glucose in dark condition. The cell size and dry weight of these species in heterotrophic condition were larger and heavier than those in phototrophic condition. The maximum culture biomass of these heterotrophic species attained to 133 mg/L/day.
Author(s)
김대근
Issued Date
2012
Awarded Date
2012. 2
Type
Dissertation
Keyword
Chlorella
Publisher
대학원 수산생물학과
URI
https://repository.pknu.ac.kr:8443/handle/2021.oak/9176
http://pknu.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000001965680
Alternative Author(s)
Dae Geun Kim
Affiliation
대학원 수산생물학과
Department
대학원 수산생물학과
Advisor
허성범
Table Of Contents
Abstract·······························································ii

Ⅰ. 서 론·······························································1

Ⅱ. 재료 및 방법··················································3
1. 타가영양성 Chlorella의 선별·································3
1. 1. Cell chamber 배양··············································3
1. 2. 250 mL플라스크 배양··········································5
2. 최적배양 환경 요인···········································6
3. 타가영양 배양 및 자가영양 배양의 성장 비교··················8
4. 통계처리·····················································8

Ⅲ. 결과·························································9
1. 타가영양성 Chlorella의 선별······································9
1. 1. Cell chamber 배양·············································9
1. 2. 250 mL플라스크 배양········································9
2. 최적배양 환경 요인············································12
2. 1. 탄소원으로 사용된 유기물 및 유기물 농도················12
2. 2. 배양 온도·······················································16
2. 3. 질소와 인 비율·················································16
3. 타가영양 배양 및 자가영양 배양 비교························19
3. 1. 세포 성장률·····················································19
3. 2. 세포 크기·······················································19
3. 3. 건조 중량·······················································23

Ⅳ. 논의························································26

Ⅴ. 결론························································31
Ⅵ. 감사의 글························································32
Ⅶ. 참고 문헌······························································33
Degree
Master
Appears in Collections:
대학원 > 수산생물학과
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