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PUKYONG Repository 대학원 해양생물학과

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Alternative Title: Distribution and feeding ecology of the file fish, Thamnaconus modestus in the southern sea of Korea

Abstract: Tamnaconus modestus was collected in the southern sea of Korea. T. modestus distribute in depth ranging 80~120 m and warmer water temperature than 12℃. The size of specimens ranged from 10.6 to 38.7 cm in total length (TL). Body length of the fish found in deeper depth was significantly larger than that of more shallow depth. To understanding feeding habits of T. modestus, stomach contents was analized. The main method of analysis in present study was analysis of stomach contents based on morphology. Molecular analysis by polymerase chain reaction (PCR) of DNA was surrogated because morphological identification accompany often difficult due to digestion and ingestion. T. modestus was omnivorous, consuming diverse prey such as algae, amphipods, gastropods, ophiuroids and cephalopods. Diet composition showed slightly ontogenetic fluctuations. Food diversity increased with increasing fish size.

URI: https://repository.pknu.ac.kr:8443/handle/2021.oak/9192
http://pknu.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000001965835

Table Of Contents: List of figures
List of tables
Abstract
1. Introduction 01
2. Materials and Methods 04
2-1. sampling 04
2-1-1. Fish sampling 04
2-1-2. Oceanographic sampling 05
2-2. Laboratory analysis 07
2-2-1. Plankton biomass 07
2-2-2. Morphological identification of stomach contents 07
2-2-3. Molecular identification of stomach contents 08
2-2-3-1. DNA extraction 08
2-2-3-2. Primer selection and PCR amplification 09
2-2-3-3. DNA sequencing 09
2-3. Data analysis 13
2-3-1. Vacuity index 13
2-3-2. Stomach contents weight 13
2-3-3. Index of relative important (IRI) and occurrence (%) 14
2-3-4. Dietary breadth index 14
2-4. Statistical analysis 15
3. Results 17
3-1. Fish abundance 17
3-2. Relationship between total length and body weight 24
3-3. Oceanographic characteristics 26
3-3-1. Depth, temperature and salinity 26
3-3-2. Biomass of zooplankton 30
3-4. Analysis of stomach contents 31
3-4-1. Size composition of Thamnaconus modestus 31
3-4-2. Vacuity index 34
3-4-3. Stomach contents index (SCI) 36
3-4-4. Prey composition 38
3-4-4-1. Prey composition by morphological identification 38
3-4-4-2. Prey composition by supplement of molecular identification 45
3-4-4-3. Prey composition in relation to fish size 3-4-5. Dietary Breadth Index 57
4. Discussion 59
5. Acknowledgement 64
6.References 66

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