비소(As) 노출에 의한 틸라피아 Oreochromis niloticus의 기관별 축적과 항산화 반응

Abstract

Abstract Arsenic is an non-essential trace element which effects toxicity to human and animals. In this study, laboratory experiments were carried out to determine the accumulation of Arsenic(As) in tilapia and the biochemical responses in liver and gill of freshwater tilapia Oreochromis niloticus under 20, 25 and 30℃ temperatures. tilapia were exposed to 200㎍/l and 400㎍/l arsenic for 10 and 20days. Accumulations in gill and liver were significantly changed with elevated temperature. These data indicate that tilapia, Oreochromis niloticus liver and gill can be considered adequate target tissues for waterborne exposured of As. blood were measured on total protein, glucose, GOT and GPT in tilapia exposed to As concentrations. Total protein and glucose were increased and the elevated activities of GOT and GPT were observed during 20days exposure period compared to those of the control group. arsenic exposed tilapia reduced the contents of glutathione and decreased activities of glutathione reductase, glutathione peroxidase in liver and gill. but, biphasic responses on GST in the tissues. It was shown that the antioxidant defense parameters of tilapia, including the contents of GSH and activities of GR, GPx, GST were sensitive to arsenic exposure, accompanied by changes of arsenic accumulation under different temperatures in liver and gill tissue. And these data indicates that tilapia, Oreochromis niloticus liver and gill can be considered adequate target tissues for waterborne exposure of As and also implied that excessive waterborne As can effect the toxicity of xenobiotics to tilapia through alterations in antioxidant system and bioaccumulation.