사염화탄소로 유도된 흰쥐의 간 손상에 대한 다시마 추출물의 보호 효과

Abstract

Laminaria japonica is a category of large seaweed plants that can grow to more than 200 feet in length in underwater forests. The brown algae contains several vitamins and minerals with many nutritional benefits and has been widely used in native diets in many countries. The liver is an organ that plays a major role in metabolizing endogenous and exogenous materials. CCl4 is a well known potent hepatotoxicant and has primarily been used as a chemical to assess hepatotoxicity in animal experiments. This study was investigated the changes in various biochemical markers related to hepatotoxicity in groups pretreated with Laminaria japonica extract(LJE) following CCl4 administration to assess the protective effect of LJE against CCl4 induced acute liver injury in rats. Experimental animals were divided into 4 groups, control, CCl4 and CCl4＋LJE 150, 300 mg/kg treatment group, with 5 rats in each group. The rats were administered orally with doses of 150, 300 mg/kg LJE for 10 days. 3 hour after the final treatment, all groups except for the control group were subjected to intraperitoneal injection with CCl4. One day later, blood and liver samples were collected to analyze biomarkers. For this study measured not only the activity of serum enzymes(GOT, GPT), which are used as indicators of hepatic injury, but also the antioxidant enzyme activities of liver tissue such as GSH content, SOD and CAT activities. In addition, EGFR expression and CCl4-induced apoptosis of the liver by oxidative stress involving changes in MAPKs were analyzed by Western blot. The following results was obtained from this experiment. The activities of GOT and GPT were significantly higher than that of the control group, indicating the damage of liver tissue by CCl4-induced. But, in the LJE groups, the increase of GOT and GPT activities were markedly decreased. The hepatic SOD activity of the group that received CCl4 alone was significantly increased. And hepatic GSH content and CAT activity of the CCl4 group were lower than in the control group, resulting in liver damage. On the other hand, the SOD activities of the LJE pretreatment groups was significantly decreased compared to the CCl4 group. GSH and CAT level of the LJE groups were significantly increased. Therefore, The increase of antioxidant activities in the LJE groups reduced oxidative stress by their scavenging effect on the ROS. EGFR responds to a diverse array of ligands, and plays important role in various cellular proliferation and invasion. MAPKs signaling cascade represents one of the intracellular signaling pathways by growth factors and oxidative stress. Western blot analysis also revealed LJE attenuated CCl4-induced EGFR and MAPKs activities in livers. In conclusion, it is identified that LJE has a protective effect against CCl4-induced hepatotoxicity.