에탄올로 유도된 흰쥐의 위 손상에 대한 클로렐라 추출물의 보호작용

Abstract

Chlorella is a unicellular microalgae that is ubiquitous in freshwater or seewater environments. Chlorella vulgaris extract (CVE) is powdered concentrate Chlorella original powder extracted the active ingredient in hot water, and is centrifuged to remove insoluble material of cells. This was used as experimental material. This study was carried out to investigate the protective effect of Chlorella vulgaris extract (CVE) on the gastric injury by ethanol in rats. Male Sprague-Dawley rats (7 weeks old) were divided into four groups and orally administered with or without for 7 days: normal group (received D·W), control group (received 6㎖ 40% ethanol/㎏ B.W.), CVE-1 group (received 40% ethanol with 150㎎ CVE/㎏ B.W.), CVE-2 group (received 40% ethanol with 300㎎ LF/㎏ B.W.). In the results of in vivo assay, the body weight and liver and stomach weight was unremarkable changed. But ethanol treatment induced gastric bleeding, surface epithelial cell destruction compared to the normal group, but co-treatment with CVE inhibited these damages. In order to determine damage of the liver tissue, the levels of GOT and GPT were slightly increased by ethanol treatments, but it was significantly decreased by treatment of CVE. In order to evaluate the anti oxidative effect of CVE in gastric tissue, the activity of SOD (superoxide dismutase) was significantly decreased by treatment of CVE compared to the control group, but the activity of GSH (Reduced glutathione) was unremarkable changed. Caspase-3 activity was increased when ethanol was injected compared to the normal group, and it was decreased by treatment of CVE. And Caspase-8 activity was the same. Taken together, this study was confirmed that CVE have beneficial effects for alcoholic gastric injury by oxidative stress and apoptosis.