톳 주정 추출물의 사염화탄소로 유발된 SD Rat의 간 손상 억제작용

Abstract

Hizikia fusiforme grows mainly in the northwest Pacific including Korea, Japan, and China, that has been widely used as food in Korea. H. fusiforme is a kind of brown seaweed that contains highly nutritious such as calcium, iron, iodin, vitamin A and various biological matters. H. fusiforme has been known to exert physiological character, diet food, antioxidant, anti-hypertension, anti-inflammatory, anti-coagulate, anti- cancer, this experiment has examined effect of H. fusiforme extracts by carbon tetrachloride-induced liver damage in SD rats. To investigate the effects of H. fusiformis extracts (HFE) on the damaged liver of CCl4-treated rats, Sprague-Dawley (SD) male rats weighing about 160g, were divided into 4 groups ; normal group (NOR), alone CCl4-treated group (CON), HFE150mg/kg BW and CCl4-treated group (HFE150), HFE300mg/kg BW and CCl4-treated group (HFE300). On 10 days and 11days, all groups except for the NOR group abdominal injection with CCl4 (CCl4: olive oil ; 1 : 1 v/v; 1 ml/kg BW). One day later, blood and liver samples were collected to analyze bio-markers. Each group were examined for the liver weight to body weight, the activities of glutamate oxaloacetate transamine (GOT), glutamate pyruvate transamine (GPT) in serum and contents of glutathione (GSH) , activities of antioxidative enzymes and caspase-3, 8 activity, DNA fragmentation in tissues. The liver weight, liver weight to body weight ratio(%) in NOR group, HFE groups were low compared to the CON group and also were not found to be significantly different among the HFE groups. In the CON group, the GOT, GPT activities increased significantly when compared with the NOR group. In contrast, the HFE groups decreased compared with CON group. The SOD and CAT activities were significantly increased in the liver tissue of rats in the HFE groups compared with the CON group. Restoration of SOD activity indicated that HFE can help in cellular defense mechanisms by preventing cell membrane oxidation. Increase in the CAT activity indicates in scavenging hydrogen peroxide. The GSH levels were not found significantly decrease in the liver tissue of rats in the HFE groups, NOR group compared with the CON group. But, the GSH levels were significantly decreased in the serum of rats in the HFE groups and NOR group compared with the CON group. An decrease in GSH content indicated that HFE also help in the restoration of vital molecules such as NAD, cytochrome and glutathione. The caspase-3, -8 activities were significantly decreased HFE groups and NOR group compared to CON group. Finally, the results indicate that the inhibition effects of HFE by carbon tetrachloride-induced liver damage in SD rats.