HaCaT 각질 세포주에서 Corydalis heterocarpa 로부터분리된 coumarin이 광손상에 미치는 효과

Abstract

Solar ultraviolet (UV) radiation, particularly its UVB (315-280 nm) element, has a variety of harmful effects on skin and leads to skin disease. The harmful biological effects of UVB includes, sunburn cell formation, melanoma, photoaging, and skin cancer via metaboliteproduction of ROS. Recently, some studies have been reported on antioxidative activities from isolated salt-tolerant plants such as corydalis heterocarpa. This study focus on investigation of anti-photodamage effects of two coumarin compounds isolated from corydalis heterocarpa; columbianetin (A) and libanoridin (B) on UVB induced human keratinocyte (HaCaT) cells. In the results it was observed that compounds A and B suppressed UVB induced, generation of reactive oxygen species (ROS) and DNA damage. And also the compounds down regulated the UVB induced protein and gene expressions of MMP-2 and MMP-9. Furthermore, formation of apoptotic sunburn cells due to excessive UVB exposure was also alternated by the compounds treatment. The under lying signaling mechanisms of UVB protective effects of compounds A and B was elucidated through ASK1-MAPK-responsive signaliling pathways.