Nonylphenol 분해 미생물의 분리 및 분해 특성

Abstract

Nonylphenol (NP), a breakdown product of nonylphenol ethoxylates (NPEs), is a subset of the alkylphenols and widely used as a industrial surfactant. This compound is classified as a xenobiotic and considered as a controversial pollutant. In the environment, NP has been detected widely in untreated sewage or waste water streams. It has been known that NP is toxic to many aquatic organisms and also well known for its endocrine system disrupting potential. Extremely low concentrations of NP would result in any detectable estrogenic effects in animals and humans. Due to its environmental concerns, it has been replaced by more expensive alcohol ethoxylates, which is less problematic environmentally. To remove toxic organic compounds, both biological and chemical treatments have been suggested as an environmental friend method. Bioremediation of the toxic compounds, using microbes or enzymes produced from the microorganism is often considered as economically and environmentally favorable method. Therefore, many efforts have been performed to isolate bacteria capable of degrading NP. However, there is no obvious report about the biodegradation of NP. With the increase of necessity for eco-friendly decomposition against toxic organic compounds, this study was conducted to isolate NP-degrading microorganism from aqueous environment. NP-degrading microbes were isolated through NP-containing enrichment culture from various sources such as sewage, waterweed, and sediment. Finally, a microbial consortium, SW-3, capable of degrading NP with high efficiency was selected from the mixture sample. Microbial consortium SW-3 was able to degrade over 99% of 100 ppm NP in the culture media for 40 days incubation at 25℃. Considering above result, It was concluded that microbial consortium SW-3 utilized NP as a single carbon source. The microbial consortium obtained in this study would be useful to develope an eco-friend bioremediation technology for NP degradation.