다시마 당단백질에 의한 에탄올 위 손상 보호작용 효과

Abstract

Abstract

As people's eating habit has been variously changing due to continuous economic growth and income increase, various chronic degenerative diseases, such as aging, cerebrovascular diseases, heart diseases, high blood pressure or diabetes, has been increased, as well as the increase of mortality. Peptic ulcer causes epigastric pain, chest pain, hyperchylia, gastro-intestinal bleeding, spasm, and pressure, sometimes with nausea, vomiting, anorexia and weight loss (Ruth, 1998). The medications to treat gastric perforation or gastric bleeding caused by gastric ulcer has been developed to treat pain and ulcerss and to avoid relapse (Higham et al., 2000), however, those medications are generally expensive and have various side effects. Therefore, there has been researches to examine materials that have pharmacological effects without any side effects by dividing bioactive substances from natural substances. Laminaria japonica is very rich in major minerals that are involved in physiological metabolism of body such as calcium, kalium, iodine, and zinc (Ayako et al., 1992), as well as dietary fibers such as alginic acid which consists of uronic acid, laminaran (Lee et al., 1998) and fucoidan (Hwang et al., 1996) that contains sulfate group. There has been reported that Capsosiphon fulvescens, Haematococcus and Sagassum fulvellum showed effects of damage inhibition by ethanol (Hwang et al, 2008; Raghavendran et al, 2004; Kamath et al, 2008), however there has not been any research on effects of Laminaria japonica. Thus, this experiment has examined the effect of glycoprotein, among the components of Laminaria japonica, on gastric damage in vivo experiments. After one week acclimation to the basal diet, S.D. white rats were divided into 3 categories-control group, ethanol injecting group, injecting ethanol and LGP group and the results of the short-term experiments are as below. According to gross morphology of inside surface of stomach, ethanol injection caused gastric bleeding, however, it inhibited bleeding just like in the control group when LGP was also injected. Caspase-3 activity was increased when ethanol was injected (144±22.6) compared to control group (100%), and it is decreased to 89.8±34.4% when 150 mg/kg B.W. was injected and to 87.6±10% when 300 mg/kg B.W. was injected. Caspase-8 activity was increased when injecting ethanol (176.4±29.3) compared to the control group (100%), and it is decreased to 128±40.1% when injecting 150 mg/kg B.W. and to 110.7±31.8% when injecting 300 mg/kg B.W. The level of proteins of Procaspase-3 is degraded and decreased in the group injected with ethanol and increased while treated by LGP, and the level of proteins of cleaved PARP is increased in the group injected with ethanol and decreased while treated by LGP. GSH is more increased when treating ethanol solely (77.2±8.60%) compared to GSH in gastric tissues of the control group, however, it is increased again in the group that LGP was also injected (150 mg/kg, 100.6±2.70%. 300 mg/kg, 104.0±7.81%). In MAPK (Mitogen-activated protein kinases) signal transmission, ERK 1/2 was increased when treating ethanol and increased when injecting with LGP (150 mg/kg B.W.), on the other hand, JNK increased phosphorylation when treating ethanol and decreased when injecting ethanol with LGP, and p38 MAPK showed same trend with ERK. The results suggest that alcohol-induced gastric damage in S.D. rats caused by oxidative damage due to ethanol is protected by treating LGP and that there is a possibility that LPG is useful for inhibiting alcohol-induced gastric damage as a functional new material.