혹서실로부터 분리한 Floridoside에 의한 mesenchymal stem cell의 조골분화효과

Abstract

Floridoside [α-D-galactopyranosyl-(1-2)- L-glycerol] from edible red alga Laurencia undulata, has known as an abundant marine biological resources. In the presents study, floridoside of Laurencia undulate have shown to promote the osteoblast differentiation in D1 mouse bone marrow mesenchymal cells. Floridoside noted to enhance phenotype markers such as alkaline phosphate (ALP), type I collagen, osteocalcin, osteopontin and mineralization. Our results indicate that floridoside stimulates osteoblast differentiation at various stages, from maturation to terminally differentially differentiated osteoblats. Induction of differentiation by floridoside is associated with increased bone morphogenetic proteins-2 (BMP-2) production. Induction of differentiation by floridoside is associated with the increased activation mRNA expression and protein expression of associated BMP/Smad pathway such as p-Smad 1/5/8, Runx2 and Osterix. This results suggests that floridoside exerts promoting effect on osteogenetic differentiation, which plausibly function via the BMP pathway. Considering to be effective candidates for applications in food and pharmaceutical fields as natural marine antioxidants.